This secondary antibody was purified from antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. The antibody made by our company is improved based on the original production. Tested by controlled experiment, the antibody has a strong reaction with the IgG heavy and light chains and low reaction with human IgG through adsorption human serum protein.
This secondary antibody was purified from antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. The antibody made by our company is improved based on the original production. Tested by controlled experiment, the antibody has a strong reaction with the IgG heavy and light chains and low reaction with human IgG through adsorption human serum protein.
Product Name:
Goat anti-Rabbit IgG (H+L)-HRP
Cat. Number:
IO-R005
Physical State：
Liquid
Application:
Western blot(WB) , ELISA
Recommended dilutions:
1:5000-1:50000 for WB with ECL substrates;
1:1000-1:10000 for ELISA and WB with chromogenic substrates
Concentration：
1mg/ml
Size：
100μl / 1ml
Storage :
It is stable at least for one year at -20°C. Avoid repeated freezing and thawing cycles. The storage buffer is 0.01M sodium phosphate with 0.25M NaCl and 50% glycerol and the pH is 7.6. The stabilizer is BSA(3mg/mL).
Note:
This product is not included with enzyme substrate reagent. This product is for research use only, not for diagnostic or therapeutic use.
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